Cytotoxic correlation with BACCOR biopharmaceutical mass spectrometry in the biocorrosive process of endodontic files

Autores

  • Fabiano Luiz Heggendorn UNIGRANRIO Post Graduate Program in Dentistry (PPGO), Grande Rio University (UNIGRANRIO). Prof. José de Souza Herdy, 1.160 C- Duque de Caxias / Rio de Janeiro, Brazil - Zip Code: 25071-202
  • Gabriela Cristina Carvalho da Silva Federal University of Rio de Janeiro School of Pharmacy, Pharmaceutical Bioassays Laboratory, Health Sciences Center, CCS, Bloco K, R050, University City, Zip Code: 21941-599, Rio de Janeiro, RJ, Brazil
  • Lúcio Souza Gonçalves Faculty of Dentistry, Estácio de Sá University - Rio de Janeiro, Av. Alfredo Baltazar da Silveira, 580, Zip Code: 22790-710, Rio de Janeiro, RJ, Brazil
  • Walter Barreiro Cravo Junior Biocorrosion and Biodegradation Laboratory, National Institute of Technology, Av. Venezuela, 82, 6th floor, Praça Mauá, Zip Code: 200 81-312, Rio de Janeiro, RJ, Brazil
  • Marcia Teresa Soares Lutterbach Biocorrosion and Biodegradation Laboratory, National Institute of Technology, Av. Venezuela, 82, 6th floor, Praça Mauá, Zip Code: 200 81-312, Rio de Janeiro, RJ, Brazil
  • Sabrina de Castro Brasil Post Graduate Program in Dentistry (PPGO), Grande Rio University (UNIGRANRIO). Prof. José de Souza Herdy, 1.160 C- Duque de Caxias / Rio de Janeiro, Brazil - Zip Code: 25071-202
  • Viviane Oliveira Freitas Lione School of Pharmacy, Federal University of Rio de Janeiro, Laboratory of Pharmaceutical Bioassays, Health Sciences Center, CCS, Block K, Room 050 University City, Zip Code: 21941-599, Rio de Janeiro, RJ, Brazil

Palavras-chave:

Desulfovibrio, oral microbiology, Sulfate-reducing bacteria, Endodontic Files.

Resumo

This study evaluated the biopharmaceutical BACCOR, developed to promote the biocorrosion of fractured endodontic files inside the root canal. The cytotoxicity of cell extracts was evaluate from supernatants and pellets, without the occurrence of biocorrosion of two species Desulfovibrio desulfuricans, oral strain and environmental strain, and Desulfovibrio fairfieldensis in different culture media. The results were correlated with the most probable number of each bacterial strain and with the analysis of chemical species obtained in energy dispersive spectrometry in RX under a scanning electron microscope. L929 and Vero cell lines were used for MTT cytotoxicity tests. Strains were inoculated into modified Postgate E culture medium (MCP-E mod), Modified Postgate E without Agar-agar (MCP-E s/Ag), Postgate C at 4 g./l. of Agar-agar (MCP-C 4.0 g/l Ag) and Postgate C without Agar-agar (MCP-C s/Ag). After the 42-day cultivation periods cell extracts from Supernatant and Cell Pellet were prepared. All MCP-C supernatants showed lower cell viability when compared to the others using MCP-E. The cell viability of the supernatants was lower than the cell viability of their respective pellets. The test compounds originated from metabolic products and cell lysates of the Sulfate-reducing Bacteria strains showed better results in the groups with MCP-E s/Ag and MCPE-mod, being classified as non-cytotoxic and with moderate cytotoxicity, according to ISO categorization, with viability cell over 50%, which makes this type of inoculum acceptable as a biomaterial. 

Biografia do Autor

Fabiano Luiz Heggendorn, UNIGRANRIO Post Graduate Program in Dentistry (PPGO), Grande Rio University (UNIGRANRIO). Prof. José de Souza Herdy, 1.160 C- Duque de Caxias / Rio de Janeiro, Brazil - Zip Code: 25071-202

Post Graduate Program in Dentistry (PPGO), Grande Rio University (UNIGRANRIO). Prof. José de Souza Herdy, 1.160 C- Duque de Caxias / Rio de Janeiro, Brazil - Zip Code: 25071-202

Gabriela Cristina Carvalho da Silva, Federal University of Rio de Janeiro School of Pharmacy, Pharmaceutical Bioassays Laboratory, Health Sciences Center, CCS, Bloco K, R050, University City, Zip Code: 21941-599, Rio de Janeiro, RJ, Brazil

Federal University of Rio de Janeiro School of Pharmacy, Pharmaceutical Bioassays Laboratory, Health Sciences Center, CCS, Bloco K, R050, University City, Zip Code: 21941-599, Rio de Janeiro, RJ, Brazil

Lúcio Souza Gonçalves, Faculty of Dentistry, Estácio de Sá University - Rio de Janeiro, Av. Alfredo Baltazar da Silveira, 580, Zip Code: 22790-710, Rio de Janeiro, RJ, Brazil

Faculty of Dentistry, Estácio de Sá University - Rio de Janeiro, Av. Alfredo Baltazar da Silveira, 580, Zip Code: 22790-710, Rio de Janeiro, RJ, Brazil

Walter Barreiro Cravo Junior, Biocorrosion and Biodegradation Laboratory, National Institute of Technology, Av. Venezuela, 82, 6th floor, Praça Mauá, Zip Code: 200 81-312, Rio de Janeiro, RJ, Brazil

Biocorrosion and Biodegradation Laboratory, National Institute of Technology, Av. Venezuela, 82, 6th floor, Praça Mauá, Zip Code: 200 81-312, Rio de Janeiro, RJ, Brazil

Marcia Teresa Soares Lutterbach, Biocorrosion and Biodegradation Laboratory, National Institute of Technology, Av. Venezuela, 82, 6th floor, Praça Mauá, Zip Code: 200 81-312, Rio de Janeiro, RJ, Brazil

Biocorrosion and Biodegradation Laboratory, National Institute of Technology, Av. Venezuela, 82, 6th floor, Praça Mauá, Zip Code: 200 81-312, Rio de Janeiro, RJ, Brazil

Sabrina de Castro Brasil, Post Graduate Program in Dentistry (PPGO), Grande Rio University (UNIGRANRIO). Prof. José de Souza Herdy, 1.160 C- Duque de Caxias / Rio de Janeiro, Brazil - Zip Code: 25071-202

Post Graduate Program in Dentistry (PPGO), Grande Rio University (UNIGRANRIO). Prof. José de Souza Herdy, 1.160 C- Duque de Caxias / Rio de Janeiro, Brazil - Zip Code: 25071-202

Viviane Oliveira Freitas Lione, School of Pharmacy, Federal University of Rio de Janeiro, Laboratory of Pharmaceutical Bioassays, Health Sciences Center, CCS, Block K, Room 050 University City, Zip Code: 21941-599, Rio de Janeiro, RJ, Brazil

School of Pharmacy, Federal University of Rio de Janeiro, Laboratory of Pharmaceutical Bioassays, Health Sciences Center, CCS, Block K, Room 050 University City, Zip Code: 21941-599, Rio de Janeiro, RJ, Brazil

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Publicado

2022-10-10

Como Citar

Heggendorn, F. L., da Silva, G. C. C., Gonçalves, L. S., Cravo Junior, W. B., Lutterbach, M. T. S., Brasil, S. de C., & Lione, V. O. F. (2022). Cytotoxic correlation with BACCOR biopharmaceutical mass spectrometry in the biocorrosive process of endodontic files. Almanaque Multidisciplinar De Pesquisa, 9(1). Recuperado de https://publicacoes.unigranrio.edu.br/amp/article/view/7325

Edição

v. 9 n. 1 (2022): Almanaque Multidisciplinar de Pesquisa

Seção

Ciências da Saúde e Meio Ambiente