Cytotoxic correlation with BACCOR biopharmaceutical mass spectrometry in the biocorrosive process of endodontic files
Palavras-chave:
Desulfovibrio, oral microbiology, Sulfate-reducing bacteria, Endodontic Files.Resumo
This study evaluated the biopharmaceutical BACCOR, developed to promote the biocorrosion of fractured endodontic files inside the root canal. The cytotoxicity of cell extracts was evaluate from supernatants and pellets, without the occurrence of biocorrosion of two species Desulfovibrio desulfuricans, oral strain and environmental strain, and Desulfovibrio fairfieldensis in different culture media. The results were correlated with the most probable number of each bacterial strain and with the analysis of chemical species obtained in energy dispersive spectrometry in RX under a scanning electron microscope. L929 and Vero cell lines were used for MTT cytotoxicity tests. Strains were inoculated into modified Postgate E culture medium (MCP-E mod), Modified Postgate E without Agar-agar (MCP-E s/Ag), Postgate C at 4 g./l. of Agar-agar (MCP-C 4.0 g/l Ag) and Postgate C without Agar-agar (MCP-C s/Ag). After the 42-day cultivation periods cell extracts from Supernatant and Cell Pellet were prepared. All MCP-C supernatants showed lower cell viability when compared to the others using MCP-E. The cell viability of the supernatants was lower than the cell viability of their respective pellets. The test compounds originated from metabolic products and cell lysates of the Sulfate-reducing Bacteria strains showed better results in the groups with MCP-E s/Ag and MCPE-mod, being classified as non-cytotoxic and with moderate cytotoxicity, according to ISO categorization, with viability cell over 50%, which makes this type of inoculum acceptable as a biomaterial.